S-STEM Scholar Anh Nguyen's Blog

  Introduction:      In lab this week, my partner Jessica Sabel and I worked on plating kanamycin with different concentrations onto TGY. Since our 96 wells plate results came back pretty decent, we decided to move onto plating the kanamycin.  Fig. 1: Results As shown here, the wells came out decent. Rows A-C (1-6) contains the kanamycin, TGY, and aquaticus. Row F contained the kanamycin and TGY (which is why there were exponential growth) and Row H only contained our blanks which were only TGY and our aquaticus.  Fig. 2: Concentration Wells Based on the results of the figure shown here, we came to the conclusion that the concentration of 1.5 ug/ml and 1.74 ug/ml are enough for aquaticus.  Process:  With that said, we laid our 10 plates of TGY which half will be the concentration of 1.5 ug/ml and the other half will be the concentration of 1.74 ug/ml. Before pouring the plates, we obtained two beakers of pure TGY and autoclaved each beakers for 30...

Introduction:        Following our previous 96 wells results of contamination, we found a foreign bacteria in our TGY which resulted in an unusual growth in our blanks and negative controls. In this week, we wanted to ensure that our antibiotic (kanamycin) and our bacteria and cell (aquaticus) is not also contaminated. Therefore, it was my job to do a gram stain and check on our bacteria to see if it is contaminated or not. This gram stain took a total of 2 tries due to some errors and mistakes. Day 1: October 31st      For the first day, I did a gram stain on both of our aquaticus which dated back to 8/16/22 and 9/30/22. The results came back quite unusual and unfortunate.  8/16/22 Cells Fig. 1: 40X Lens                                                            ...