Week 5: More Plasmid Extractions
Introduction: Because of the lack of success from our plasmid extractions, we will be conducting more plasmid extraction but this time experimenting with different variables and independent factors that could've altered our results. As before, some of our plasmid extraction came out pretty good in terms of the concentration rate but unfortunately, did not show up well or even at all on a gel. Therefore, we will be redoing everything but with different independent factors. On 3/02/23, my partners and I decided to experiment with 4 different tubes, each having different procedures and its own factors, and we will see the different types of results. We have: --> Tube A: follows every step except the lysozyme procedure and no buffer (just get straight into the Zymopure Plasmid Kit), we also need to take aliquots of each wash (1+2+2) and put it into the nano drop. --> Tube B: follows every step but this time adding the lysozyme and lysis procedure (lysozy...