Week 13 (4/1-4/5): Repeating transformation with pwTCas9 plasmid and D.caeni
Introduction In lab this week, we repeated a transformation protocol with the pwtCas9 plasmid and D. caeni hoping that it can give us good results on plates to do plasmid confirmation. The beginning of the week comprise of growing plasmid culture in 20 mL of LB broth with 50 ug/ml of ampicillin. We also prepared TGY agar plates with 2ug/ml of tetracycline to observe our transformed cell growth. We hope that there will be transformed cells grown on the plates as the pwtCas9 plasmid was proven to be resistant to tetracycline. Plasmid Extraction Procedure Below is a list comprising of how we performed plasmid extractions with pwtCas9. Solutions # of Cell Packing Resuspension Elusion Buffer Amt PC1 1 ...