Week 2: Combining fragments with Tetr/a and Deinococcus fragments

Week 2: Combining Tet and Fragments

     In the lab this week, we worked with the combination of Tetr/a and Deinococcus fragments. 


Procedure: 

    To start, it is important to understand how the experiment was started and the concept of it. I included sketches of what it would look like. 




As shown here, we have our primers: 
FRAGMENT 1
    - P1
    - P2
TET
    - P3
    - P4
FRAGMENT 2
    - P5 
    - P6


Each are made from Deinococcus while Tet, as shown, was taken and sampled from E. Coli. The goal here is to put Fragment 1 (P1-P2) and Fragment 2 (P5-P6) into parts of Tet. This explains the "split" line between Tet. However, it shouldn't be confused that Tet is being split in half; just Tet is being given to each fragments.

To emphasize on that idea, here is a more specific example of how the fragments are all each going to be combined into Tet

This is a more specific idea and concept. As shown, Fragment 1 is being combined with Tet 1 while Fragment 2 is being combined with Tet 2. 











After being mixed and combined, Fragment 1 and Tet 1 will be LEFT TET while Fragment 2 and Tet 2 will be RIGHT TET. 
As shown here, this is the mixture with LEFT TET and RIGHT TET each having 4 tubes. 













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