Week 2: Quick Innoculation

Introduction: 

    In lab this week, my group and I used the plates we've made and used a grown sample of D. Aquaticus to inoculate into the plates. 

Procedure: 

    Before we inoculate the plates, we had to prepare our D. Aquaticus and its growth in order to transfer it. We got 2 flask with 40 mL of TGY Media and grew our bacteria in it. We then took gram stains of each flasks to see for any contamination but thankfully, it was am abundant of growth in our media. We decided to do 4 plates. First in our inoculation, we decided to burn the transfer loop before touching any culture/bacteria to avoid contamination. Then, we put the loop into the first 40mL flask and transfer it to 2 plates by streaking,  consisting heat fixing both the loop and the mouth of the flask each time when it comes in closely with bacteria or other media. We repeated this for the second 40mL flask but with the rest of the 2 plates. Lastly, once we were finished, we put all 4 plates in the 30 C incubator since D. Aquaticus in that temperature range. We also remember to sanitize everything in order to prevent easy contamination. We will check back next week for any growth or progress.

Discussion: 

    Even though this week was simple and just consisted of growing bacteria, gram staining, and inoculation, we expect the next few weeks to be consistently busy because if there is good growth and no contamination in our plates and media, we can go forward in plasmid extraction using the GeneJET Plasmid Miniprep Kit. If the plates turn out not very good and have a slight contamination, then we would have to restart the growth and inoculation process all over again. 

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