S-STEM Scholar Anh Nguyen's Blog

 Introduction In lab this week, my group and I did one more testing with Tetracycline and Caeni. However, based on the results last week, we decided to do the concentrations below 1 ug/ml. The concentrations are 0 ug/ml, 0.25 ug/ml, 0.5 ug/ml, and 0.75 ug/ml. We expect at least some growth between 0 ug/ml and 0.5 ug/ml.  Procedure  To prep, we made four 25ml flasks of normal TGY agar. The reason for this is because we want to ensure that each TGY plates we will be pouring is evenly 25ml. We then recieved our 2.5 ug/ml tetracycline stock from Chad. We also grew our caeni in a 10ml TGY flask.  To keep in mind, our OD value of caeni was 2.44 after 3-4 days of growth and was diluted to 0.97. 1. We label each of four 25ml flasks of TGY agar their desired tetracycline concentrations and also labeled four empty plates the corresponding concentrations to the flasks.                    Flask #1 and Plate #1: 0ug/ml...

Introduction     In lab this week, my group and I will repeat the tetracycline and caeni experience once more. However, since we learned that both caeni and d.aquaticus showed that it is resistant to tetracycline in no more than the concentration of 10ug/ml, we will be redoing that experiment with caeni but now with only the concentrations of 0ug/ml, 1ug/ml, 2ug/ml, and 6ug/ml. Going into this, we want to expect at least growth on all plates especially between the concentrations 0ug/ml to 2 ug/ml.  Procedure     The prepping for week is different than last weeks because instead of working with soft agar and mixing it in with the tetracycline, we will be working with normal agar. To prep, we made four 25ml flasks of normal TGY agar. The reason for this is because we want to ensure that each TGY plates we will be pouring is evenly 25ml. We then recieved our 2.5 ug/ml tetracycline stock from Chad. We also grew our caeni in a 10ml TGY flask.  To keep in mind, a...

Introduction  This week in lab, we checked out the results of our aquaticus and tetracycline plates from week 3. Since we weren't able to do caeni  with tetracycline in week 3, we planned to do it with the exact protocol that we used to experiment with  d.aquaticus to see if caeni will show the same results as d.aquaticus when it stresses with tetracycline.  Results Figure 1 D.Aquaticus and Tetracycline plate results  Shown here are the plates of D. Aquaticus and tetracycline. From 10ug/ml to 1000ug/ml (1 mg/ml), there is absolutely no growth while 0ug/ml has substantial growth but 0ug/ml plates do not have any antibiotics. This tells us that D. Aquaticus  is not resistant to tetracycline and show no substantial resistance from 10 ug/ml to 1000 ul/ml. Thus we can assume that D. Aquaticus may show some resistance within the 0ug/ml and 10 ug/ml concentrations but it is not significant enough since those concentrations may be too small.  Figure 2 Caeni an...