1/22-2/3 Week 4: Tetracycline Efflux Pump Results and Caeni with Tet.

Introduction 

This week in lab, we checked out the results of our aquaticus and tetracycline plates from week 3. Since we weren't able to do caeni with tetracycline in week 3, we planned to do it with the exact protocol that we used to experiment with d.aquaticus to see if caeni will show the same results as d.aquaticus when it stresses with tetracycline. 

Results

Figure 1 D.Aquaticus and Tetracycline plate results 

Shown here are the plates of D. Aquaticus and tetracycline. From 10ug/ml to 1000ug/ml (1 mg/ml), there is absolutely no growth while 0ug/ml has substantial growth but 0ug/ml plates do not have any antibiotics. This tells us that D. Aquaticus  is not resistant to tetracycline and show no substantial resistance from 10 ug/ml to 1000 ul/ml. Thus we can assume that D. Aquaticus may show some resistance within the 0ug/ml and 10 ug/ml concentrations but it is not significant enough since those concentrations may be too small. 





Figure 2 Caeni and Tetracycline plate results 


Shown here are the plates of Caeni and tetracycline. Just like the D. Aquaticus results, there is absolutely no growth from the 10 ug/ml to 1000 ug/ml plates but there's substantial growth from the 0 ug/ml plates. We can also conclude that Caeni also show low resistance to tetracycline. 

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